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    LAB DATA:

    https://www.northallegheny.org/cms/lib/PA01001119/Centricity/Domain/1235/Yeastdatacmbl.cmbl

    https://www.northallegheny.org/cms/lib/PA01001119/Centricity/Domain/1235/yeastdatacsv.csv

     

    Open Inquiry Student Directed Investigations
    Lab Experimental Plan Due by: Thurs Nov 19

    For student-directed investigations, it is expected that the student will provide an experimental plan for the teacher to approve before beginning any experiment. The general plan format follows that of writing a grant to fund a research project.

    Each lab partner should create the following plan for the experiment that you choose in their notebook and title it Enzyme Reactions Inquiry Lab Plan.

    1. Define the question or testable hypothesis.

    2. Describe the background information. Include previous experiments.

    3. Describe the experimental design with controls, variables, and observations.

    4. Describe the possible results and how they would be interpreted.

    5. List the materials and methods to be used.

    6. Note potential safety issues.

    One lab partner must submit the group’s plan for approval prior to conducting the experiment.  No two groups in any sections can do the exact same experiment.  Therefore, the sooner you get me your plan the better the odds that you will not be denied the opportunity of conducting your dream investigation!

    Over the last few weeks we have discussed a number of biological concepts/processes involving enzymes that we have talked about in class or explored in a lab setting.  Look over the experiments that you have recorded in your lab notebook.  Consider the ideas that are included under “Extensions”.  Decide on an interesting factor that you would like to further explore.  You are encouraged to do research to learn more about different enzymes and the reactions that they catalyze.

    Model your investigation around this title:
    The effect of  “insert some condition” on the enzyme  “insert enzyme name” during “insert a description of the reaction”.

    For example this was an extension that we further explored in the Enzyme lab:
    The effect of enzyme concentration on reaction rate during the decomposition of hydrogen peroxide by catalase.

    Enzyme ideas:
    Catalase (beef, turnip, yeast extraction)
    Lactase (Lactaid tablets)
    Amylase (saliva, germinating corn seeds, Diastase malt powder lab grade)
    Protease (Papain – pineapple juice/meat tenderizer, Pepsin powder lab grade)
    Alcohol Fermentation enzymes – baker’s yeast
    Respiration enzymes – germinating seeds, small insects (small intact living/growing organisms)
    Photosynthesis enzymes – fresh leaves
    Light reaction enzymes – leaf pigment extraction

    Consider the following:

    The effect of XXXXX on YYYYYY: (How does X affect the enzymes in Rxn Y?)
    YOUR OPTIONS ARE NOT LIMITED TO THESE AND OBVIOUSLY MANY OF THESE TERMS CAN BE MIXED AND MATCHED J

    Independent XXXXX

    Dependent YYYYYY

    Temperature

    Gas pressure

    pH

    Respiration

    Salinity

    Photosynthesis

    Substrate Concentration

    Rate of Reaction

    Food Type (Sugar, protein, fat)

    Formation of CO2

    Color of Light (for photosynthesis)

    Disappearance of O2

    Enzyme concentration – can’t be catalase for this

    Fermentation

    Cell type ( compare diff plants, animals, fungi)

    Digestion/Hydrolysis

     

    THE BEST DESIGNS HAVE GOOD CONTROLS IN PLACE AND A RELIABLE METHOD TO RECORD AND ANALYZE DATA.

    When approached to discuss your plan, the members of your lab group should be able to:

            Explain the purpose of a procedural step.

            Identify the independent variables and the dependent variables in your experiment.

            Predict and justify the results that you would you expect to see in the control group?  The experimental group?

            How does XXXX concept affect YYYY findings?

            Describe the method to determine XXXX.

    ·         Explain the method quantitatively or qualitatively by which you will assess YYYY.

    Revisit the Labs that we have completed thus far and consider some ideas that you may want to further to investigate.

    Complete the assignment on Masteringbiology and check the following labs in your Google Folder for completion:

    Student Labs:

    Alien invasion

    Termite Taxi response

    Animal behavior/choice:

    Milky Rainbows

    Eukaryotic cells

    Protein Assay Lab: Sample data to make graphs from, curve fit and predictions about unknowns

    Label

    Concentration (mg/mL)

    Absorbance

    1

    .125

    .65

    2

    .250

    .69

    3

    .500

    .97

    4

    .750

    1.15

    5

    1.000

    1.08

    6

    1.500

    1.57

    7

    2.000

    1.45

    Milk

    unknown

    1.44

    HP

    unknown

    1.53

     
    Week of 9/28/2015:
    Chapter 4: A Tour of the cell and Eukaryotic Cell Lab
    Chapter 4 Assignments are on www.masteringbiology.com 
    Please visit the tabs on the left for notes, assignment help, and links 
     
     
    Official College Board AP Exam Information Site:
     
     
Last Modified on November 16, 2015