Lab #6 Molecular Biology / E.coli Bacterial Transformation Conclusion Guidelines: Please answer these questions in paragraph form. The completed conclusion questions will be turned in on Tuesday Feb. 12th stapled to your labs.
pGreen Transformation- What is transformation? How can you induce competence (cause transformation)?
- What bacteria was used? What genes are on the pGreen plasmid?
- What was the difference between the prepared + and - cell suspensions?
- Which plates were the controls? Which plate was the experimental? Describe and explain the differences in the growth patterns on all 4 plates.
- What is transformation efficiency? What factors might influence this number?
- Describe any mistakes during your lab that might have affected the results of this experiment.
Last Modified on February 8, 2013